This document is a valuable working tool for anyone who works in the health networks of Latin American countries. It describes the most recent basic and fundamental guidelines for the diagnosis and treatment of American trypanosomiasis (Chagas disease) based on the evidence published to date, and is now being put into the hands of all interested parties by the Pan American Health Organization (PAHO). The work carried out by a team of Chagas disease specialists in coordination with experts in the GRADE methodology (Grading of Recommendations Assessment, Development and Evaluation), provides the highest guarantees and scientific credibility, giving health workers and patients clinical knowledge that is based on the most up-to-date and reliable evidence and knowledge available. Chagas disease is a neglected infectious disease that affects between six and eight million people in the Americas. Current estimates indicate that there are roughly 28,000 new acute cases each year, and nearly 65 million people live at continuous risk of contracting the disease by vector-borne transmission, blood or congenital transmission, or food-borne transmission. For these reasons, PAHO recognizes that there are substantial needs in terms of increasing access, coverage, and quality of care within national health care systems, mainly in primary care networks. This document is without question a significant contribution to the training of new health workers. We hope that it will effectively contribute to basic and refresher training for all healthcare personnel in the public and private sectors, and that it will help standardize the required knowledge and procedures for the diagnosis and treatment of this endemic parasitosis. These guidelines were developed as part of the Chagas-related commitments made by the PAHO Directing Council in Resolution CD55. R9 (2016): Plan of Action for the Elimination of Neglected Infectious Diseases and Post-Elimination Actions, 2016-2022.